Cooperativity and Interaction Energy Threshold Effects in Recognition of the −10 Promoter Element by Bacterial RNA Polymerase
نویسندگان
چکیده
منابع مشابه
Cooperativity and interaction energy threshold effects in recognition of the −10 promoter element by bacterial RNA polymerase
RNA polymerase (RNAP) melts promoter DNA to form transcription-competent open promoter complex (RPo). Interaction of the RNAP σ subunit with non-template strand bases of a conserved -10 element (consensus sequence T-12A-11T-10A-9A-8T-7) is an important source of energy-driving localized promoter melting. Here, we used an RNAP molecular beacon assay to investigate interdependencies of RNAP inter...
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The key step in bacterial promoter opening is recognition of the!10 promoter element (T!12A!11T!10A!9 A!8T!7 consensus sequence) by the RNA polymerase s subunit. We determined crystal structures of s domain 2 bound to single-stranded DNA bearing !10 element sequences. Extensive interactions occur between the protein and the DNA backbone of every!10 element nucleotide. Base-specific interactions...
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The key step in bacterial promoter opening is recognition of the -10 promoter element (T(-12)A(-11)T(-10)A(-9)A(-8)T(-7) consensus sequence) by the RNA polymerase σ subunit. We determined crystal structures of σ domain 2 bound to single-stranded DNA bearing-10 element sequences. Extensive interactions occur between the protein and the DNA backbone of every -10 element nucleotide. Base-specific ...
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15 صفحه اولA third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase.
A DNA sequence rich in (A+T), located upstream of the -10, -35 region of the Escherichia coli ribosomal RNA promoter rrnB P1 and called the UP element, stimulates transcription by a factor of 30 in vivo, as well as in vitro in the absence of protein factors other than RNA polymerase (RNAP). When fused to other promoters, such as lacUV5, the UP element also stimulates transcription, indicating t...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2013.11.4473